Publication number |
CLPUB00136 |
Authors |
Kim H.J., Song M.J., Kim D.H., Kang E.S., Jin O.Y., Kim S.H., Kim J.H., Kim Y.T., Kim J.W. |
Title |
Establishment of a novel malignant Brenner tumor cell line. |
Citation |
Korean J. Obstet. Gynecol. 48:2321-2329(2005) |
Web pages |
https://www.komci.org/GSResult.php?RID=0021KJOG%2F2005.48.10.2321&DT=1&ITEMNO=10&TITEM=45https://ir.ymlib.yonsei.ac.kr/bitstream/22282913/151335/1/T200501255.pdf |
Abstract |
Objective: Ovarian cancer is the most lethal disease among gynecologic
malignancies. Although many efforts have been made to explore the
mechanisms involved in its development, the genetic events in the
pathogenesis of ovarian cancer are still unclear. We characterized a cell
line (designated OHK) established from a malignant Brenner tumor cell.
Methods: The cells were obtained during the operation of a 43-year-old
Korean woman with ovarian cancer. The OHK cells continuously propagated in
vitro over a period of about 36 months and, to date, have undergone over
200 passages, without being infected by either Mycoplasma or any bacteria.
We measured the doubling time of OHK cells. To investigate the
tumorigenecity of OHK, cells were inoculated subcutaneously into the back
of nude mice. Several tumor markers were analyzed using culture media and
lysates of cytosol. Morphology and ultrastructure were analyzed by phase-
contrast microscopy and electron microscopy. OHK was also analyzed for
gene mutation, the typing of human leukocyte antigen and Flow cytometric
cell cycle analysis and DNA index.
Results: They proliferated in a monolayered sheet showing a pavement-like
arrangement without suppression by intercellular contacts. They also
formed epithelial cell lining in shapes of polymorphism and polygons.
Doubling time was 38.4 hour which was relatively slow compared to other
cancer cells. Microscopic view revealed intranuclear infoldings which are
typical in malignant Brenner tumors. The OHK cells secreted significantly
high level of CA 125 into the culture medium. A 215th codon at exon 4 of
p53 was mutated to C/C in OHK. BRCA1 was a wild type and polymorphisms
were detected in exons 2, 10, 11, 14 and 17 of BRCA2. The cells showed
aneuploidy with DNA index of 1.589 measured by flow cytometry. When
transplanted into nude mice, OHK cells successfully induced tumor which
was histopathologically resembled malignant Brenner tumor.
Conclusions: These results strongly suggest that OHK is a typical cell line
of malignant Brenner tumor. This may provide a useful cellular resource
for studying the pathogenesis of malignant Brenner tumor.
|
Cell lines |
CVCL_W731; OHK [Human ovarian tumor] |