Publication number |
CLPUB00129 |
Authors |
Schlage W.K., Kuhn C., Bereiter-Hahn J. |
Title |
Established Xenopus tadpole heart endothelium (XTH) cells exhibiting selected properties of primary cells. |
Citation |
Eur. J. Cell Biol. 24:21-21(1981) |
Abstract |
Endothelial cells from Xenopus laevis tadpole hearts spread extremely flat
on glass surfaces. This property makes them a favourite source for
studying intracellular dynamics i.e. motility of mitochondria. For a
combination of light microscopy methods with physiological measurements
like oxygen consumption we established a permanent cell line which
preserved the main morphological properties of the primary cells. Such a
line was derived from dispase-treated hearts of stage 53 tadpoles. After
15 generations a stationary phase was reached. From cells which overcame
this phase several sublines have been established. Their altered growth
behaviour and chromosomal variations ascertain the transformed status of
the XTH cells.
|
Cell lines |
CVCL_1892; XTH-2 |