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Cellosaurus publication CLPUB00129

Publication number CLPUB00129
Authors Schlage W.K., Kuhn C., Bereiter-Hahn J.
Title Established Xenopus tadpole heart endothelium (XTH) cells exhibiting selected properties of primary cells.
Citation Eur. J. Cell Biol. 24:21-21(1981)
Abstract Endothelial cells from Xenopus laevis tadpole hearts spread extremely flat on glass surfaces. This property makes them a favourite source for studying intracellular dynamics i.e. motility of mitochondria. For a combination of light microscopy methods with physiological measurements like oxygen consumption we established a permanent cell line which preserved the main morphological properties of the primary cells. Such a line was derived from dispase-treated hearts of stage 53 tadpoles. After 15 generations a stationary phase was reached. From cells which overcame this phase several sublines have been established. Their altered growth behaviour and chromosomal variations ascertain the transformed status of the XTH cells.
Cell lines CVCL_1892; XTH-2