| Publication number |
CLPUB00119 |
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| Authors |
Wang S.-L., Li F., Pu P.-Y., Wang G.-X., Peng Q., Wang C.-Y. |
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| Title |
The establishment and characteristics of human glioblastoma cell lines TJ899 and TJ905. |
|---|
| Citation |
Tianjin Yiyao 24:416-418(1996) |
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| Web pages |
https://www.cqvip.com/qk/92492X/199607/2218099.html |
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| Abstract |
The author established in vitro cell lines derived from two glioblastomas
of the occipital and occipital regions of the human cerebral palsy. It has
survived for 5 years and passed over 500 generations. Methods and Results
1. Establishment of cell lines: 1. Source of material: TJ899 cell line was
obtained from a surgically removed specimen of a male patient with
occipital glioma. The TJ905 in vitro cell line was obtained from a male
TOP glioma patient. Both were diagnosed as glioblastoma multiforme by
histopathology. 2. Construction: After aseptic conditions, the tissue was
rinsed with Hanks' solution to remove blood and visible electrocautery and
necrotic tissue. Mechanically fragmented, inoculate the tissue block in a
25cm~2 flask, add Waymouth's MAB 87/3 (Gibco Lab, USA) containing 15%
fetal bovine serum (FCS), incubate at 37 Celsius, 5% CO2 72 hour. Most
tissue blocks have different degrees of cell migration, and the cell body
is small. After 1 week, most of the culture flask walls are full of cells.
|
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| Cell lines |
CVCL_B264; TJ899 CVCL_6975; TJ905 |
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