Publication number |
CLPUB00099 |
Authors |
Karunasagr I., Miller S.D., Frerichs G.N. |
Title |
A new cell line from Puntius schwanenfeldi sensitive to snakehead fish cell line C-type retrovirus. |
Citation |
Asian Fish. Sci. 8:151-157(1995) |
Web pages |
http://www.asianfisheriessociety.org/publication/abstract.php?id=904 |
Abstract |
A new cell line was established from the caudal peduncle of a healthy,
juvenile warmwater cyprinid, Puntius schwanenfeldi. The cell line has been
designated PSP. Cultures grew well in Leibovitz L-15 medium supplemented
with 10% fetal calf serum at 25-30 Celsius and have been passaged more
than 60 times since initiation over 2.5 years ago. The PSP line was
refractory to infection with a range of viruses associated with outbreaks
of the epizootic ulcerative syndrome (EUS), including infectious
pancreatic necrosis virus (IPNV) serotype Sp and Ab, and goby birnavirus
and snakehead rhabdovirus, but was found to be sensitive to C-type
retrovirus released from a persistently infected striped snakehead
(Ophicephalus striatus) cell line.
|
Cell lines |
CVCL_S010; PSP [Puntius] |