| Abstract |
Fibroblast-like cells obtained from explant culture of an one-day-old fry
of a naturally occurring gynogenetic teleost, the Amazon molly
(Poeciliaformosa), were serially subcultivated with a doubling time of
approximately 1.5 days at 27 Celsius for 540 days when the cells attained
252 Population Doubling Number (PDN) and the serial passage was stopped.
No symptom of crisis was encountered during the passage. At several PDNs,
changes in various biological parameters were examined. The growth
characteristics of the cells strongly indicated that this PF line, like
some other cell lines of fish, appears to have unlimited proliferating
potentials without any apparent sign of being malignantly transformed, and
hence to have been immortalized without transformation. During
subcultivation, the cloning efficiencies considerably fluctuated between
approx. 1 to 20%. Although the number of chromosomes was the same (46) at
12, 75, and 175 PDNs, all of the 46 chromosomes were acrocentric at 12 PDN
while 45 acrocentric and 1 submetacentric chromosomes were found at 75 and
175 PDNs. The saturation cell density ranged from 6.8 to 12x10^4
cells/cm^2 during passage and the contact inhibition of growth generally
worked well. The cells were highly proficient in photoreactivation of
UV-induced cell killing as well as DNA damage in terms of UV-specific
endonuclease sensitive sites. The potential usefulness of this cell line
for study of relationships between DNA damage and carcinogenesis was
discussed.
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