| Abstract |
We have previously reported that owl monkeys (Aotus trivirgatus) are hosts
for several different latent viruses. Most of these viruses have been
isolated from kidney cell cultures. To characterize these herpes, adeno,
paramyxo, and papova viruses recovered as latent agents from the owl
monkey, there was an urgent need for an established owl monkey kidney cell
line which was free of adventitious agents. We were successful in
obtaining such a cell line which is now in its 258th passage level. The
chromosome analysis of the cell line at different passage intervals by the
trypsin G-band technique demonstrated that the cell line was initiated
from a female Aotus with a "type II" karyotype and a diploid count of 54.
The cell line remained hypo-diploid with a modal count of 51 to 53.
Drastic rearrangement of parts of certain chromosomes has been noted. This
cell line permits the replication of several herpesviruses, especially the
two oncogenic viruses H. saimiri and H. ateles as well as several other
mammalian viruses. This cell line is also useful for plaquing studies. It
will withstand the photodynamic action of neutral red for as long as 34
days and permit the development of viral plaques. The morphological,
chromosomal, and biological characteristics of this cell line will be
discussed.
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