| Publication number |
CLPUB00092 |
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| Authors |
Etoh H., Suyama I., Hyodo-Taguchi Y., Matsudaira H. |
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| Title |
Establishment and characteristics of various cell lines from Medaka (Teleostei). |
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| Citation |
(In book chapter) Invertebrate and fish tissue culture; Kuroda Y., Kurstak E., Maramorosch K. (eds.); pp.266-269; Japan Sci. Soc.; Tokyo; Japan (1988) |
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| Web pages |
https://www.springer.com/de/book/9783642736285 |
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| Abstract |
Four permanent cell lines, OLME-104 from melanoma of HB32C medaka, OLHE-131
from hepatoma of HO4C, OLF-136 from the normal fins of HB32C and OLF-124
from those of outbred medaka of orange-red variety, were established. From
their growth curves, doubling times were estimated to be 43 h for OLF-136,
77 h for OLME-104 and 67 h for OLHE-131. Nearly diploid amounts of
DNA for OLF-136 and OLME-104, but nearly tetraploid for OLHE-131 cells
were observed on cytofluorometrical analysis and confirmed on metaphase
preparations. The cultured cells were injected ip into respective host
fish. No nodule was observed in HB32C medaka injected with OLF-136 cells.
The fish injected with OLME-104 and with OLHE-131 cells died within a
month due to tumor growth. The OLME-104 cells were transplantable to HB32C
medaka but not to HO4C and outbred medaka. The OLHE-131 cells were
transplantable only to HO4C. The tumor cells have maintained their
malignant characters for many generations in in vitro culture. It seems
from UV-irradiation experiments that these cell lines from medaka, as well
as a cell line from goldfish, are more sensitive to UV-radiation compared
to the cultured mammalian cells.
|
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| Cell lines |
CVCL_R980; OLF-124 CVCL_3766; OLF-136 CVCL_4873; OLHE-131 CVCL_3767; OLME-104 |
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