| Abstract |
During the last two years we have carried out studies on cell cultures
from pre-invasive and invasive carcinoma of the uterine cervix. One of our
objects has been to establish new cell strains. The culture material
consisted of 380 specimens from 96 patients. We have succeeded in
establishing four new strains, originating from four patients. They are
designated NHIK 3016, NHIK 3017, NHIK 3025 and NHIK 3043 and were started
in September 1967, October 1967, November 1967 and April 1968,
respectively. Biopsies were taken under coloscopic guidance and cut
longitudinally, one half for histological examination; the remaining part
for in vitro studies. The histological diagnoses of the biopsies were
invasive carcinoma for NHIK 3016 and NHIK 3043 and hyperplasia for NHIK
3017 and NHIK 3025. The corresponding clinical diagnoses were invasive
carcinoma for NHIK 3016, NHIK 3017 and NHIK 3043 and carcinoma in situ for
NHIK 3025. The tissue specimens, consisting of both epithelium and stroma,
were minced and trypsinized to single cells and cultivated in Carrel
flasks containing Puck's medium E2a. During the first 3 to 4 months the
mitotic activity was moderate; then a sudden increase was observed. At
this stage the cells had uniform epithelial-like morphology and growth
pattern. These properties have remained unchanged and the cells continue
to show permanent high mitotic activity.
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