| Abstract |
NB-I is a continuous and functional cell line of hypodipolidy, bearing two
marker chromosomes typical of this line, obtained from metastatic neck
lymph node of a human neuroblastoma. The cells were exponentially
proliferated in 20% fetal calf serum in Eagle's MEM, in floating and/or
attached types. This line grew in soft agar media. Plating efficiency
increased in paraller with cell numbers in inoculum. The floating cell
were ligh-refractile, spherical in shape, with tendency to cluster and
estimated to be a blast type. The attached cells made up fine mesh works
on glass surface connected each other with slender processes, defined as a
differentiated and mature type. Growth curves of both cells were somewhat
different, i.e., the former grew rapidly and the latter slowly. The
generation time of both cells in toto showed Tg = 29.6 hr, Ts = 13.2 hr by
means of 3H-TdR autoradiography. The cell processes were stained brownish
in color by Bodian's method and electromicroscopic studies revealed that
they contained small number of microtubules. The paperchrom tographic
investigation of cultured media, treated by 3H-tyrosine in advance,
indicated that DOPA and HVA was synthesized in cells in vitro.
Histochemically, cultured cells revealed positive catecholamine
fluorescense and monoamine oxidase activity. It is concluded that this
line is derived from malignant sympathetic neurons.
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