| Publication number |
CLPUB00085 |
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| Authors |
Minowada J., Oshimura M., Abe S., Greaves M.F., Janossy G., Sandberg A.A. |
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| Title |
Human leukemia cell lines. Evidence for differentiation toward T- and B-cell axis within a leukemia. |
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| Citation |
Proc. Am. Assoc. Cancer Res. 19:109.434-109.434(1978) |
|---|
| Abstract |
Blood cultures from a 19 y.o. male patient with a non-T,non-B acute
lymphoblastic leukemia in relapse gave rise to 8 "leukemia" and 2 "normal-
B" cell lines. At the time of culture (Nov.'76), the membrane phenotype of
his leukemic blasts consisted of SmIg (lambda and mu chain
immunoglobulins), Ia-like B cell associated antigen (Ia) and common form
of ALL specific antigen (cALL), despite a typical non-T,non-B ALL
phenotype at the disease onset in Oct.'75. Among 8 established leukemia
cell lines (NALM 6-13), there exist some differences in profiles of the
membrane phenotype. Except B85 and B86, all NALM lines represent the
leukemia cells on the basis of the presence of the cALL antigen and two
common marker chromosomes (5q- and 12p+) which had been present in the
original uncultured leukemia cells as well. Variations in membrane
phenotype expression seen in this study, despite the presence of common 2
cytogenetic markers and monoclonoal SmIg (lambda, mu) suggest that intra-
clonal differentiation of the leukemia clone could take place to a limited
extent.
|
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| Cell lines |
CVCL_WW76; B85 CVCL_0092; NALM-6 CVCL_5677; NALM-7 CVCL_L057; NALM-8 CVCL_L058; NALM-9 CVCL_L059; NALM-10 CVCL_L060; NALM-11 CVCL_5660; NALM-12 CVCL_L061; NALM-13 |
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