| Abstract |
Cultured lymphoid tumor cell lines (BMC-L2-1(C2), BMC-L2-1(C10), and
BMC-L2-20(C1)) and non-cultured transplantable cell line (BMC-TL2)
were derived from the same individual with Marek's disease experimentally
produced in the syngeneic line BM-C chicken (MHC: BA/BA). Then, the
established cell lines were examined of their characteristics. The
results were summarized as follows. 1) T-cell antigen and MHC BA antigens
were respectively expressed on all of the cells of the 3 cultured cloning
cell lines. 2) Brightly stained MATSA were observed on the long term
cultured cell lines (BMC-L2-1(C2) and BMC-L2-1(C10)) using indirect
immune-fluorescence technique. On the other hand, MATSA was negative on
the non-cultured transplantable cell line (BMC-TL2). When cultured in
vitro for a long term. however, MATSA of the cell line was gradually
expressed on the cell membranes. 3) Isologous transplantations were
carried out using the cell line BMC-L2-20(C1) cultured from the
transplantable cell line (BMC-TL2). The transplantations were
successful, following transfer of the tumor cells to the internal organs,
only in the case of the inoculation of the cultured tumor cell (MATSA was
negative) of the early stages of cultivation. However, according to the in
vitro culture for long period (over 6 months), the transplantabilities of
the tumor cells were gradually decreased with the marked appearance of the
MATSA. 4) Efficiencies of colony formation in soft agar of the cell lines
of BMC-L2-1(C2), BMC-L2-1(C10) and BMC-L2-20(C1) were 97%, 63%, and 12%,
respectively.
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