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Cellosaurus publication CLPUB00072
| Publication number | CLPUB00072 |
|---|---|
| Authors | Sato H., Yoshida M.C., Sekiguchi T. |
| Title | Q-banded karyotypes of a 6-thioguanine resistant rat cell line and its mutant line resistant to chloramphenicol. |
| Citation | Chrom. Inform. Serv. 27:17-18(1979) |
| Web pages | https://eurekamag.com/research/029/013/029013421.php |
| Abstract | L6TG, a 6-thioguanine resistant derivative of the original L6 line of rat myoblasts (Yaffe, 1968), is deficient in hypoxanthine phosphoribosyl- transferase. L6TG which is resistant to 100 micro g/ml chloramphenicol (CAP) is a mutant isolated from the above mentioned L6TG line. This double mutant line provides a new selection system in somatic cell hybridization experiments, since hybrid cells between this mutant and normal, CAP sensitive, cells can grow in the HAT selective medium containing CAP, whereas the parental cells die (Sekiguchi, unpublished). We analyzed the chromosomes of L6TG and L6TGcapr cells by means of our routine air-dry method with a combined quinacrine mustard and 33258 Hoechst staining. |
| Cell lines | CVCL_C565; L6TG CVCL_C566; L6TG Cap |