| Abstract |
Attempts to develop sensitive tissue culture systems for isolation of
possible viral agents responsible for epidemic gastroenteritis have led to
the establishment of five serially transplantable cell lines from one
human embryo. The organs of origin included brain, lung, heart, liver and
intestine. An epithelial-like morphology was established after 3-5 serial
transfers and has persisted for one year. Multinuclear giant cells are
frequently observed with nuclei numbers ranging from 3-20. The spectrum of
susceptibility to known enteroviruses varies with each cell line.
Isolation of the cell line, the viral spectrum and morphological
characteristics will be discussed.
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