| Abstract |
A continuous cell line was established from the pectoral fin of a jarbua,
Therapon jarbua Forskal. The cell, designated JF, have been passed for 90
times during 9 months in Leibovitz's L-15 plus 10% foetal bovine serum
under a temperature of 28 +- 1 Celsius. The cell line consists of
fibroblastic cells with dipolar or polypolar. Chromosome analysis
indicates the cells are heteroploid and show a modal numbers of 48. A low
plating efficiency of approximately 1.7% was recorded for JF cell at 28 +-
1 Celsius. The temperature ranged from 18 to 37 degree Celsius, the pH
from 6 to 8 or the osmolarity from 200 to 600 mmol/kg could support the
growth of JF cell line. JF cells were also demonstrated to the susceptible
to Infectious Pancreatic Necrosis Virus (IPNV) AB, S, VR-299 serotypes and
Chum Salmon Virus (CSV).
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