| Publication number |
CLPUB00051 |
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| Authors |
Ishikawa I., Soma M., Ono I., Nakaguchi T., Ishawata C., Nozawa S., Ishikawa H. |
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| Title |
Biological characteristics of adenocarcinoma cell line (HOUA-I) and B-lymphoblastoid cell line (HOUA-II) established from the same tissue of endometrial adenocarcinoma. |
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| Citation |
Jikeikai Med. J. 36:303-315(1989) |
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| Web pages |
https://jglobal.jst.go.jp/en/detail?JGLOBAL_ID=200902086883036321 |
|---|
| Abstract |
Human endometrial adenocarcinoma was cultured and endometrial
adenocarcinoma cell line (HOUA-I) and B-lymphoblastoid cell line (HOUA-II)
were established. HOUA-I line consists of cells of spindle-like, roundish,
and polygonal shapes, arranged in epithelial pavement and proliferating in
multilayers. Their doubling time is about 50 hours and plating efficiency
is about 25%. The chromosomes show a wide aneuploid distribution, with a
mode at 46, and no karyological abnormality. The cells can be transplanted
on hamster and nude mouse to form poorly differentiated adenocarcinoma.
The HOUA-I line was determined as the endometrial adenocarcinoma cell line.
On the other hand, HOUA-II line consists of small roundish cells with
cytoplasmic processes, and the cells proliferate as floating spherical
aggregates of cells. Their doubling time is 60 hours, and the chromosomes
of the stem cells are 46,XX,inv (20p+q-). They give positive results for
Epstein-Barr virus nuclear antigen (EBNA) but negative result for EB-viral
capsid antigen (EBVCA). Synthesis of immunoglobulin G (IgG) and c-myc gene
amplification were recognized. The HOUA-II line was determined as
B-lymphoblastoid cell line.
|
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| Cell lines |
CVCL_3867; HOUA-I CVCL_A778; HOUA-II |
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