| Publication number |
CLPUB00050 |
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| Authors |
Sekiya S., Kawata M., Iwasawa H., Takamizawa H. |
|---|
| Title |
Establishment of a human teratocarcinoma cell line of the ovary (HOTC3) and its cell biological characteristics. |
|---|
| Citation |
Nihon Sanka Fujinka Gakkai Zasshi 33:2249-2249(1981) |
|---|
| Web pages |
https://ci.nii.ac.jp/naid/110002209233/en |
|---|
| Abstract |
We established a human teratocarcinoma cell line of the ovary (HOTC3) and
compared its cell biological characteristics with other human cell lines
derived from various gynecologic tumors such as ovarian adenocarcinoma
(HOC-21), carcinoma of the uterine endometrium (HEC-1B), squamous cell
carcinoma of the uterine cervix (SKG-1), and choriocarcinoma (BeWo). The
results are as follows. 1) BeWo, HOC-21 and HOTC3 cells revealed high
activity of intracellular LDH in this order, however none of HEC-1B and
SKG-1 cells did. 2) The activity of intracellular Al.p. was higher in BeWo
cells than in HOC-21 cells. The isozyme of Al.p. detected in these cells
were found to be heat-stable. The others revealed no activity of Al.p. 3)
The presence of hCG-beta was confirmed in both BeWo and HOTC3 cells. The
intracellular levels of hCG-beta were found to be higher in BeWo cells
than in HOTC3 cells. The gonadotropin was observed to leak into culture
medium not from HOTC3 cells but from BeWo cells. It was not detected in
the other cultured cells. 4) No AFP was detected in any of these five cell
lines. 5) Plasminogen activator was detected in HOC-21, HEC-1B and SKG-1
cells in contrast to HOTC3 and BeWo cells which were negative for
plasminogen activator.
|
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| Cell lines |
CVCL_8707; HOTC-3 |
|---|
