| Abstract |
A cell line (HKA-1) derived from a keratoacanthoma occurring on human skin
has been established in vitro, and proliferation successfully maintained
in a continuous tissue culture for about 3 and a half years. The tumor was
clinically and histologically diagnosed as a giant keratoacanthoma. The
cells grow in a monolayer and are epithelioid with anaplastic features.
Chromosome analysis of HKA-1 showed hypertriploidy with the modal number
78 at the 35th passage. The doubling time was about 34 h at the 37th
passage. The nude mouse transplantation was not successful. Analysis of
cytokeratin from HKA-1 by two-dimensional gel electrophoresis revealed
polypeptides No. 5, 6, 14, 16 and 17. The cell line is available to other
investigators.
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