| Abstract |
By using the technic of tissue culture we have successfully established a
strain of complex of epithelial-like and fibroblast-like cells from the
snout tissue of grass carp (Ctenopharyngodon idellus), and it is denoted
as strain ZC-7901. The medium used in the cultivation is "199"
supplemented with 15% fetal bovine serum containing a mode-rate amount of
antibiotics. The incubation temperature is at 25-28 Celsius. A substrain
of single epithelial-like cells is also obtained from ZC-7901 cells of
11th generation, which is named substrain ZC-7901S1. At present both the
strain and substrain have been maintained in proliferation for over 22
months through 120 and 90 generations respectively, and the cells of them
are able to reproduce with approximately 1.5x10^5 cells per millilitre in
concentration. About 4 days, a monolayer cells can be found clearly in the
culture. After 36 hours, the index of mitotic division of the ZC-7901 is
36.5% while that of the ZC-7901S1 is 32.3%; and then at 156 hours after
propagation the former is gradually decreased to 12.3% and the latter to
14.3%. The chromosome number of each cell in these two strains is 2n = 48.
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