| Publication number |
CLPUB00030 |
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| Authors |
Kasai H., Yoshimizu M. |
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| Title |
Establishment of two Japanese flounder embryo cell lines. |
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| Citation |
Hokkaido Daigaku Suisan Kagaku Kenkyu Ihou 52:67-70(2001) |
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| Web pages |
https://hdl.handle.net/2115/21946 |
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| Abstract |
Two flounder cell lines; hirame natural embryo cells (HINAE) and clone
hirame embryo cells (CHIE) were established from hirame (Japanese flounder
Paralichthys olivaceus) embryos. Both cells were cultured using a
Leibovitz's L-15 medium at 20 deg C. HINAE and CHIE cells consisted of
fibroblastic cells. Doubling time of HINAE and CHIE cells were 9 and 12
days, respectively. HINAE and CHIE cells showed cytopathic effects (CPE)
when infectious pancreatic necrosis virus (IPNV), infectious hematopoietic
necrosis virus (IHNV) and hirame rhabdovirus (HIRRV) were inoculated. Both
cells did not show a susceptibility to flounder herpesvirus (FHV),
causative agent of viral epithelial hyperplasia and Japanese flounder
nervous necrosis virus (JF-NNV). HINAE cells showed a susceptibility to
Japanese flounder lymphocystis disease virus (JF-LCDV) and produced
enlarged rounded cells. Infectivity of culture grown JF-LCDV using the
HINAE cells reached 10^5.30 TCID50/ml. HINAE is a first cell line to
support a growth of JF-LCDV.
|
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| Cell lines |
CVCL_R885; CHIE CVCL_R908; HINAE |
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