| Abstract |
Cultivation of human cancer in vitro provides an efficient tool for cancer
research and is very useful for morphological and biochemical analysis of
cancer at cellular level. Therefore, many attempts have been made to
culture human cancer cells, including adenocarcinoma cells of the
alimentary tract. However only a little success has been reported on the
establishment of human cancer cell line. This may be mainly due to some
technical difficulties for cultivation, e.g., bacterial and fungous
contaminations of inocula and overgrowth of normal fibroblasts as well as
macrophages in primary culture, and also adaptability of human cancer
cells in vitro. Taking these into consideration, the present authors have
tried to cultivate cancer cells from metastatic foci of 8 patients with
the primary colorectal carcinomas, and succeeded in long-term cultivation
of one rectal carcinoma. The present report deals with successful
establishment of a human rectal adenocarcinoma cell line (CaR-1) and its
characterization.
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