| Abstract |
A new fibroblastic cell line was established from the caudal fin of the
goldfish, Carassius auratus. The cells, designated CAF have been
subcultured over 80 times since their initiation in August 1977. There was
no difference in the growth rate between 10 and 20% fetal calf serum. At
5% fetal calf serum and 20% calf serum, the growth curves fell slightly
lower than the 10 and 20% levels of fetal calf serum. CAF cells were
cultured at various temperatures. The rate of cell growth increased with
increasing temperature over the range of 16 to 33 Celsius, whereas no cell
growth was observed at 6 Celsius. Plating efficiency of CAF cells was
estimated to be 10%, when 2,000 or more cells per dish were inoculated at
26 Celsius.
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