| Abstract |
Fourteen permanent cell lines have been established from normal tissues of
seven species of warm-water fish cultured in Taiwan. All the cell lines
were routinely grown in Leibovitz's L-15 medium supplemented with 10-20%
foetal calf serum at incubation temperatures ranged 28-31 Celsius. Amongst
these lines, EO-2 and OGN are fibroblastic and TK-1, T0-2, TM, BR, FI, GI,
CF, LF, SK, BGF are epithelioid in morphology. However, both epithelioid
and fibroblastic cells were observed in GON and EK-1 cell lines. the cell
lines were demonstrated to be free of mycoplasmal, fungal and bacterial
contaminations. With the exception of LF cells, thirteen cell lines were
susceptible to IPNV of various serotypes, CV, LV-1, EVE, EVA and EVEX.
Application of cell lines to the study of fish virology, cell physiology,
biochemistry and toxicology are discussed.
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