| Abstract |
A transplantable tumor line derived from hyperplastic nodules of the
exocrine pancreas from a rat treated with azaserine (Longnecker et al.,
Canc. Lett. 7, 197, 1979) has been used to isolate two distinct cell
culture lines. Intraperitoneal and subcutaneous tumors in a male Wistar
rat were used as source material. Minced tumor fragments were seeded to
culture vessels in F12K supplemented with horse and/or fetal bovine sera.
Epithelial-like outgrowths were predominant and fibroblastic contaminants
were selected against by penicylinder ringing. One derived line AR4-2J is
highly refractile, contains significant amounts of amylase (6.22 U/mg cell
protein) and other exocrine enzymes, and readily produces tumors in
athymic mice (5/5 in 30 days). The line contains few zymogen droplets and
apparently is not responsive to pancreozymin (0.2 U/ml). The second cell
line AR4-IP developed after passaging supernatant culture fluids obtained
at 24 hours after the initial seeding. These cells are non-refractile,
grow in monolayer culture with typical cobblestone appearance, are low in
amylase and other exocrine enzymes, and do not produce tumors when
injected into nude mice. The lines may be of use in comparative studies
requiring tumorigenic and non-tumorigenic cell lines from similar source
tissue.
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