| Abstract |
A new cultured cell line (ACL-15) was established from a transplantable
rat colon cancer strain induced by 1,2-dimethyl hydrazine (DMH).
Histologically, the primary colonic tumor was a poorly differentiated
adenocarcinoma; all transplanted tumors showed the same histologic
features. The doubling time of the ACL-15 cell line population was 24 h,
and the saturation density was 5.6x10^5 cells/cm2 in culture. The
chromosome number and DNA content of the ACL-15 cells were near tetraploid.
ACL-15 cells frequently metastasized to the liver and lungs in vivo.
Thirty percent of those rats inoculated with ACL-15 cells into the
subserosa of the cecum and all rats which received intrasplenic
inoculations of ACL-15 cells developed hepatic metastases. All rats
injected with ACL-15 into subcutis and into the tail vein developed
multiple pulmonary metastases. This newly established cell line, ACL-15,
can serve for a model of metastatic colonic cancer.
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