| Abstract |
The rat cell line 3Y1 (Kimura et al., 1975) has many characteristics of
normal fibroblastic cells; it has the diploid karyotype (Yamashita et al.,
1980), a high requirement for serum (Kimura, 1975; Kimura and Itagaki,
1975), and high sensitivity to density-dependent inhibition of
proliferation (Kimura et al., 1975; Okuda and Kimura, 1978). 3Y1 cells
have been useful in the study of proliferation control in cell culture
(Okuda and Kimura, 1978, 1982, and 1983; Okuda et al., 1983; Ohno et al.,
1984), in transformation experiments with a variety of viral agents
(Kimura et al., 1975; Hama and Kimura, 1978; Ishibashi et al., 1980;
Kawai, 1980; Ohno and Kimura, 1984) and a chemical carcinogen (Sandmeyer
et al., 1981), and in somatic cell genetics (Ohno et al., 1984). Sublines
of 3Y1 deficient in thymidine kinase (ATP: thymidine 5'-
phosphotransferase, EC 2.7.1.75; TK) should be useful in gene-transfer
experiments and cell hybridization.
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