| Abstract |
1618K was derived from a male with a primary retroperitoneal embryonal
carcinoma (EC) who presented, with pulmonary nodules, a large abdominal
mass, an alpha-fetoprotein (APP) of 275 ng/ml, and a human chorionic
gonadotropin (hCG) of 3800 mIU/ml. The testes were normal. Chemotherapy
with 4 cycles of cisplatin, bleomycin and vinblastine (PBV) induced a
partial remission. Further chemotherapy with P,B, etoposide, and
Adriamycin caused a transient complete remission. After 9 cycles of
cisplatin-based chemotherapy, a cytoreductive thoracotomy on 1/27/81
yielded EC. The specimen was seeded in flasks containing mitomycin C-
treated human embryonic lung fibroblast feeder cells (HEL-FFC). The cell
monolayer morphology was similar to other human EC cell lines. The cells
have undergone 60+ in vitro passages and grow well off feeder cells. The
cells produce AFP and hCG. Cell surface antigen analysis reveals stage
specific embryonic antigen-3 (SSEA-3), consistent with typical human EC
and SSEA-1, consistent with the presence of differentiated cells.
Morphological changes occur when the cells are seeded at low cell density.
Four of 12 athymic mice inoculated with 1618K developed tumors after a
median of 50 days. AFP and hCG were detected in the serum of tumor-bearing
mice (mean AFP.695 ng/ml, mean hCG=375 mIU/ml). Cells off HEL-FFC produced
EC in mice while cells on HEL-FFC produced tumors containing EC plus
teratoma (well-differentiated epithelial cells arranged in'glands).
Conclusions: 1) 1618K provides an in vitro comparison of gonadal and
extragonadal EC and may provide a model for the study of cisplatin-
resistance in human EC. 2) 1618K cells maintained on HEL-FFC retain
multipotentiality as evidence by antigenic analysis and by in vivo somatic
differentiation.
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