| Abstract |
Background and objective: Cancer cell line is the basic material of
various cancer research. Diverse cancer cell lines from various head and
neck regions are needed for biologic research of head and neck cancer.
However, cell lines derived from head and neck cancer are not common.
Recently, we have established and characterized a novel human squamous
carcinoma cell line, PNUH-12, from the hypopharynx.
Materials and methods: Among trials of twenty cases of head and neck
cancer, we established only one specimen succeeded culture passage over 50.
We characterized the cell line as follows: growth pattern and curve,
morphology using phase contrast microscope and transmission
electromicroscope, chromosomal analysis, flow cytometric analysis,
tumorigenecity by xenograft of cell line into nude mouse and morphological
comparison, expression of cytokeratin, epithelial membrane antigen and
vimentin, and P53 mutation and its sequencing.
Results: PNUH-12 showed typical growth pattern of cancer cell line,
representative morphological characteristics of squamous epithelial cell
origin, multiple numerical and structural clonal abnormalities of
chromosome, aneuploidy pattern of flow cytometry, and strong expression of
cytokeratin. The formed tumor of nude mouse showed the identical
histopathological phenotype (squamous cell carcinoma) of the original
tumor of patient and similar morphology of PNUH-12. There was one point
mutation of 78th base, C to G, in exon 7 of P53 gene.
Conclusions: PNUH-12 can be a good control material and successfully
bestowed to researchers for study of biology in head and neck cancer.
There still needed more head and neck cancer cell lines from various
regions and diverse cell types in future.
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