| Publication number |
CLPUB00002 |
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| Authors |
Ruckert F., Werner K., Aust D., Hering S., Saeger H.-D., Grutzmann R., Pilarsky C. |
|---|
| Title |
Establishment and characterization of six primary pancreatic cancer cell lines. |
|---|
| Citation |
Austin J. Cancer Clin. Res. 2:1055.01-1055.05(2015) |
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| Web pages |
http://austinpublishinggroup.com/cancer-clinical-research/fulltext/cancer-v2-id1055.php |
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| Abstract |
Background: Pancreatic ductal adenocarcinoma is an aggressive tumor;
treatment remains a challenge because of the lack of effective therapeutic
strategies. Basic research in this field is dependent on the availability
of model systems. New pancreatic cancer cell lines are therefore important
for the study of its biology. In the present study, we report the
establishment and characterization of six new pancreatic cancer cell lines
(PaCaDD-141, -159, -161, -165, -183, -188).
Material and methods: All cell lines were derived from pancreatic ductal
adenocarcinomas by the Dresden outgrowth protocol. The six cell lines
originated from different sample locations. We characterized the cell
lines by examining their morphology and their cytostructural and
functional profiles.
Results: All cell lines were cultured in optimized Dresden-medium. The
doubling time ranged from 20 to 43 hours. KRAS mutations were detected in
four of the six cell lines. Immunohistochemical staining showed
cytoplasmic expression of CK8/18, mostly membrane and partially
cytoplasmic expression of E-cadherin and strong expression of ezrin in all
cell lines. Three cell lines showed nuclear p53 accumulation and
heterogeneous expression of vimentin. SMAD4 was heterogeneously expressed
in the cell lines.
Conclusions: We were able to establish six new primary pancreatic
carcinoma cell lines. As applicable tools for basic research, these cell
lines might contribute to a better understanding and treatment of this
aggressive tumor.
|
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| Cell lines |
CVCL_M464; PaCaDD-141 CVCL_M465; PaCaDD-159 CVCL_M466; PaCaDD-161 CVCL_M467; PaCaDD-165 CVCL_M468; PaCaDD-183 CVCL_M469; PaCaDD-188 |
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