Cellosaurus HEK293 Actinin-M-cpstFRET (CVCL_ZV89)
| Cell line name | HEK293 Actinin-M-cpstFRET |
|---|---|
| Synonyms | HEK293 M-cpstFRET |
| Accession | CVCL_ZV89 |
| Resource Identification Initiative | To cite this cell line use: HEK293 Actinin-M-cpstFRET (RRID:CVCL_ZV89) |
| Comments | Characteristics: Used to report forces in actinin in living cells. Has one actinin molecule with the cpstFRET sensor incorporated between spectrin repeat domains 3 and 4 toward the middle of actinin. The cpstFRET (circularly permuted stretch sensitive FRET) sensor consists of a pair of tandemly connected circularly permutated CFP/YFPs (cpCerulean/cpVenus). Mechanical loading twists the dipole orientation from near parallel to diagonal, decreasing FRET efficiency. Genetic integration: Method=Transfection; Gene=HGNC; HGNC:163; ACTN1. Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo. Transformant: NCBI_TaxID; 28285; Adenovirus 5. Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113. |
| Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) |
| Hierarchy | Parent: CVCL_0045 (HEK293) |
| Sex of cell | Female |
| Age at sampling | Fetus |
| Category | Transformed cell line |
| Publications | PubMed=25422450; DOI=10.1073/pnas.1411683111; PMCID=PMC4267376 |
| Cross-references | |
| Cell line collections (Providers) | Kerafast; EBU024 |
| Encyclopedic resources | Wikidata; Q98126661 |
| Entry history | |
| Entry creation | 02-Jul-2020 |
| Last entry update | 19-Dec-2024 |
| Version number | 6 |