Cellosaurus HEK293 Actinin-C-cpstFRET (CVCL_ZV88)
| Cell line name | HEK293 Actinin-C-cpstFRET |
|---|---|
| Synonyms | HEK293 C-cpstFRET |
| Accession | CVCL_ZV88 |
| Resource Identification Initiative | To cite this cell line use: HEK293 Actinin-C-cpstFRET (RRID:CVCL_ZV88) |
| Comments | Characteristics: Has one actinin molecule attached to the C-terminal of cpstFRET, leaving the other end of the probe dangling free and the probe devoid of tension (force-free control). The cpstFRET (circularly permuted stretch sensitive FRET) sensor consists of a pair of tandemly connected circularly permutated CFP/YFPs (cpCerulean/cpVenus). Mechanical loading twists the dipole orientation from near parallel to diagonal, decreasing FRET efficiency. Genetic integration: Method=Transfection; Gene=HGNC; HGNC:163; ACTN1. Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo. Transformant: NCBI_TaxID; 28285; Adenovirus 5. Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113. |
| Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) |
| Hierarchy | Parent: CVCL_0045 (HEK293) |
| Sex of cell | Female |
| Age at sampling | Fetus |
| Category | Transformed cell line |
| Publications | PubMed=25422450; DOI=10.1073/pnas.1411683111; PMCID=PMC4267376 |
| Cross-references | |
| Cell line collections (Providers) | Kerafast; EBU025 |
| Encyclopedic resources | Wikidata; Q98126659 |
| Entry history | |
| Entry creation | 02-Jul-2020 |
| Last entry update | 19-Dec-2024 |
| Version number | 6 |