Cellosaurus HEK293 cpstFRET-Actin (CVCL_ZV86)
| Cell line name | HEK293 cpstFRET-Actin |
|---|---|
| Synonyms | HEK293 cpA; Beta-C-HEK |
| Accession | CVCL_ZV86 |
| Resource Identification Initiative | To cite this cell line use: HEK293 cpstFRET-Actin (RRID:CVCL_ZV86) |
| Comments | Characteristics: Has one actin molecule attached to the C-terminal of cpstFRET, leaving the other end of the probe dangling free and the probe devoid of tension (force-free control). The cpstFRET (circularly permuted stretch sensitive FRET) sensor consists of a pair of tandemly connected circularly permutated CFP/YFPs (cpCerulean/cpVenus). Mechanical loading twists the dipole orientation from near parallel to diagonal, decreasing FRET efficiency. Genetic integration: Method=Transfection; Gene=HGNC; HGNC:132; ACTB. Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo. Transformant: NCBI_TaxID; 28285; Adenovirus 5. Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113. |
| Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) |
| Hierarchy | Parent: CVCL_0045 (HEK293) |
| Sex of cell | Female |
| Age at sampling | Fetus |
| Category | Transformed cell line |
| Publications | PubMed=25422450; DOI=10.1073/pnas.1411683111; PMCID=PMC4267376 |
| Cross-references | |
| Cell line collections (Providers) | Kerafast; EBU023 |
| Encyclopedic resources | Wikidata; Q98126662 |
| Entry history | |
| Entry creation | 02-Jul-2020 |
| Last entry update | 19-Dec-2024 |
| Version number | 6 |