Cellosaurus HEK293T HA-PEROXO (CVCL_ZM07)
| Cell line name | HEK293T HA-PEROXO |
|---|---|
| Synonyms | HEK-293T HA-PEROXO |
| Accession | CVCL_ZM07 |
| Resource Identification Initiative | To cite this cell line use: HEK293T HA-PEROXO (RRID:CVCL_ZM07) |
| Comments | Characteristics: Expresses PEROXO-Tag, a chimeric protein consisting of three HA epitopes attached to the N-terminus of mEGFP and a segment of PEX26 fused to the EGFP C-terminus. This tag allows for localization to and insertion into the peroxisomal membrane. Genetic integration: Method=Transfection/transduction; Gene=HGNC; 22965; PEX26 (Note=With p.Met1_Val236del). Genetic integration: Method=Transfection/transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP). Genetic integration: Method=Transfection; Gene=UniProtKB; P03070; SV40 large T antigen. Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo. Transformant: NCBI_TaxID; 28285; Adenovirus 5. Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) (Note=tsA). Omics: Quantitative peroxisome proteome analysis by proteomics. Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113. |
| Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) |
| Hierarchy | Parent: CVCL_0063 (HEK293T) |
| Sex of cell | Female |
| Age at sampling | Fetus |
| Category | Transformed cell line |
| Publications | PubMed=32417403; DOI=10.1016/j.isci.2020.101109; PMCID=PMC7254474 |
| Cross-references | |
| Encyclopedic resources | Wikidata; Q98126676 |
| Proteomic databases | PRIDE; PXD018880
PRIDE; PXD018918 |
| Entry history | |
| Entry creation | 02-Jul-2020 |
| Last entry update | 10-Sep-2024 |
| Version number | 8 |