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Cellosaurus HEK293T Control-PEROXO (CVCL_ZM06)

[Text version]
Cell line name HEK293T Control-PEROXO
Synonyms HEK-293T Control-PEROXO
Accession CVCL_ZM06
Resource Identification Initiative To cite this cell line use: HEK293T Control-PEROXO (RRID:CVCL_ZM06)
Comments Characteristics: Expresses Control-PEROXO-Tag, a chimeric protein consisting of three Myc epitopes attached to the N-terminus of mEGFP and a segment of PEX26 fused to the EGFP C-terminus.
Genetic integration: Method=Transfection/transduction; Gene=HGNC; 22965; PEX26 (Note=With p.Met1_Val236del).
Genetic integration: Method=Transfection/transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
Genetic integration: Method=Transfection; Gene=UniProtKB; P03070; SV40 large T antigen.
Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo.
Transformant: NCBI_TaxID; 28285; Adenovirus 5.
Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) (Note=tsA).
Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0063 (HEK293T)
Sex of cell Female
Age at sampling Fetus
Category Transformed cell line
Publications

PubMed=32417403; DOI=10.1016/j.isci.2020.101109; PMCID=PMC7254474
Ray G.J., Boydston E.A., Shortt E., Wyant G.A., Lourido S., Chen W.W., Sabatini D.M.
A PEROXO-Tag enables rapid isolation of peroxisomes from human cells.
iScience 23:101109.1-101109.18(2020)

Cross-references
Encyclopedic resources Wikidata; Q98126675
Proteomic databases PRIDE; PXD018880
PRIDE; PXD018918
Entry history
Entry creation02-Jul-2020
Last entry update10-Sep-2024
Version number7