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Cellosaurus SEES3-1V Emerald, clone1 (CVCL_ZK54)

[Text version]
Cell line name SEES3-1V Emerald, clone1
Synonyms 1V-90515-6A
Accession CVCL_ZK54
Resource Identification Initiative To cite this cell line use: SEES3-1V Emerald, clone1 (RRID:CVCL_ZK54)
Comments Characteristics: When doxycycline is added the transfected Emerald (EmGFP) gene and lacZ are expressed.
Characteristics: Transposed with a PB-PuroR-Ins-tetO2-EmGFP-IRES-bgeo-PB where PB is a piggyBac sequence, Ins is a chicken HS4 insulator, ORF is the transposed human gene and bgeo is a neo-LacZ fusion protein.
Characteristics: Transfected with a pG-flox-CAGrtTA3G-IRES-Hyg-pA construct that contains a reverse tetracycline-controlled transactivator (rtTA), a fusion between a mutated version of E.coli TetR and the activating domain of HSV-1 VP16.
Genetic integration: Method=PiggyBac transposition; Gene=FPbase; GLAD6; Emerald (Note=EmGFP, green fluorescent protein derived from GFP).
Genetic integration: Method=PiggyBac transposition; Gene=UniProtKB; P00722; Escherichia coli beta-galactosidase (lacZ).
Genetic integration: Method=PiggyBac transposition; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
Genetic integration: Method=PiggyBac transposition; Gene=UniProtKB; P00552; Transposon Tn5 neo.
Genetic integration: Method=Transfection; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
Genetic integration: Method=Transfection; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR.
Genetic integration: Method=Transfection; Gene=UniProtKB; P06492; Human herpesvirus 1 VP16 (UL28) (Note=With p.Met1_Arg360del).
Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
Cell type: Embryonic stem cell; CL=CL_0002322.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_ZJ91 (SEES3-1V)
Sex of cell Male
Age at sampling Blastocyst stage
Category Embryonic stem cell
Publications

PubMed=32433964; DOI=10.1016/j.celrep.2020.107655
Nakatake Y., Ko S.B.H., Sharov A.A., Wakabayashi S., Murakami M., Sakota M., Chikazawa-Nohtomi N., Ookura C., Sato S., Ito N., Ishikawa-Hirayama M., Mak S.S., Jakt L.M., Ueno T., Hiratsuka K., Matsushita M., Goparaju S.K., Akiyama T., Ishiguro K.-i., Oda M., Gouda N., Umezawa A., Akutsu H., Nishimura K., Matoba R., Ohara O., Ko M.S.H.
Generation and profiling of 2,135 human ESC lines for the systematic analyses of cell states perturbed by inducing single transcription factors.
Cell Rep. 31:107655.1-107655.23(2020)

Cross-references
Encyclopedic resources Wikidata; Q98129500
Entry history
Entry creation02-Jul-2020
Last entry update10-Sep-2024
Version number8