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Cellosaurus Tango EDG4-bla U2OS (CVCL_ZK37)

[Text version]
Cell line name Tango EDG4-bla U2OS
Accession CVCL_ZK37
Resource Identification Initiative To cite this cell line use: Tango EDG4-bla U2OS (RRID:CVCL_ZK37)
Comments Population: Caucasian.
Characteristics: The target GPCR is linked to a TEV protease cleavage site and a GAL4-VP16 transcription factor. When activated the GPCR recruits the beta-arrestin/TEV protease fusion protein, the TEV protease cleaves off the transcription factor that translocates to the nucleus to activate the expression of the bla gene.
Characteristics: Stably expresses a beta-arrestin/TEV protease fusion protein and a beta-lactamase reporter gene under the control of an upstream activator sequence (UAS).
Genetic integration: Method=Transfection/transduction; Gene=HGNC; 3168; LPAR2.
Genetic integration: Method=Transfection/transduction; Gene=HGNC; 712; ARRB2.
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P04386; Yeast GAL4 (Note=DNA-binding domain).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P06492; Human herpesvirus 1 VP16 (UL28) (Note=With p.Met1_Arg360del).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P04517; Tobacco etch virus (TEV) protease (Note=AA 2038-2279).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P62593; Escherichia coli beta-lactamase bla (Note=With p.Met1_Phe22del, p.Ala23Met, p.Val82Ile and p.Met184Val).
Derived from site: In situ; Bone, tibia; UBERON=UBERON_0000979.
Sequence variations
  • Mutation; HGNC; 9277; PPM1D; Simple; p.Arg458Ter (c.1372C>T); ClinVar=VCV002445214; Zygosity=Heterozygous (from parent cell line).
Disease Osteosarcoma (NCIt: C9145)
Osteosarcoma (ORDO: Orphanet_668)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_ZJ93 (Tango GPCR-bla U2OS)
Sex of cell Female
Age at sampling 15Y
Category Cancer cell line
Web pages https://www.thermofisher.com/order/catalog/product/K1442
Publications

PubMed=19726785; DOI=10.1177/1087057109343809
Wetter J.A., Revankar C., Hanson B.J.
Utilization of the Tango beta-arrestin recruitment technology for cell-based EDG receptor assay development and interrogation.
J. Biomol. Screen. 14:1134-1141(2009)

Cross-references
Encyclopedic resources Wikidata; Q98133114
Entry history
Entry creation02-Jul-2020
Last entry update10-Sep-2024
Version number8