Cellosaurus Tango TBXA2R-bla U2OS (CVCL_ZK26)
| Cell line name | Tango TBXA2R-bla U2OS |
|---|---|
| Accession | CVCL_ZK26 |
| Resource Identification Initiative | To cite this cell line use: Tango TBXA2R-bla U2OS (RRID:CVCL_ZK26) |
| Comments | Population: Caucasian. Characteristics: The target GPCR is linked to a TEV protease cleavage site and a GAL4-VP16 transcription factor. When activated the GPCR recruits the beta-arrestin/TEV protease fusion protein, the TEV protease cleaves off the transcription factor that translocates to the nucleus to activate the expression of the bla gene. Characteristics: Stably expresses a beta-arrestin/TEV protease fusion protein and a beta-lactamase reporter gene under the control of an upstream activator sequence (UAS). Genetic integration: Method=Transfection/transduction; Gene=HGNC; 11608; TBXA2R. Genetic integration: Method=Transfection/transduction; Gene=HGNC; 712; ARRB2. Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P04386; Yeast GAL4 (Note=DNA-binding domain). Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P06492; Human herpesvirus 1 VP16 (UL28) (Note=With p.Met1_Arg360del). Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P04517; Tobacco etch virus (TEV) protease (Note=AA 2038-2279). Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P62593; Escherichia coli beta-lactamase bla (Note=With p.Met1_Phe22del, p.Ala23Met, p.Val82Ile and p.Met184Val). Derived from site: In situ; Bone, tibia; UBERON=UBERON_0000979. |
| Sequence variations |
|
| Disease | Osteosarcoma (NCIt: C9145) Osteosarcoma (ORDO: Orphanet_668) |
| Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) |
| Hierarchy | Parent: CVCL_ZJ93 (Tango GPCR-bla U2OS) |
| Sex of cell | Female |
| Age at sampling | 15Y |
| Category | Cancer cell line |
| Web pages | https://www.thermofisher.com/order/catalog/product/K1549 |
| Cross-references | |
| Encyclopedic resources | Wikidata; Q98133166 |
| Entry history | |
| Entry creation | 02-Jul-2020 |
| Last entry update | 10-Sep-2024 |
| Version number | 8 |