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Cellosaurus SEES3-3L (CVCL_ZJ90)

[Text version]
Cell line name SEES3-3L
Synonyms 3L
Accession CVCL_ZJ90
Resource Identification Initiative To cite this cell line use: SEES3-3L (RRID:CVCL_ZJ90)
Comments Characteristics: Transfected with a pG-flox-CAGrtTA3G-IRES-Hyg-pA construct that contains a reverse tetracycline-controlled transactivator (rtTA), a fusion between a mutated version of E.coli TetR and the activating domain of HSV-1 VP16.
Genetic integration: Method=Transfection; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
Genetic integration: Method=Transfection; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR.
Genetic integration: Method=Transfection; Gene=UniProtKB; P06492; Human herpesvirus 1 VP16 (UL28) (Note=With p.Met1_Arg360del).
Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
Cell type: Embryonic stem cell; CL=CL_0002322.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_1Q91 (SEES3)
Sex of cell Male
Age at sampling Blastocyst stage
Category Embryonic stem cell
Publications

PubMed=32433964; DOI=10.1016/j.celrep.2020.107655
Nakatake Y., Ko S.B.H., Sharov A.A., Wakabayashi S., Murakami M., Sakota M., Chikazawa-Nohtomi N., Ookura C., Sato S., Ito N., Ishikawa-Hirayama M., Mak S.S., Jakt L.M., Ueno T., Hiratsuka K., Matsushita M., Goparaju S.K., Akiyama T., Ishiguro K.-i., Oda M., Gouda N., Umezawa A., Akutsu H., Nishimura K., Matoba R., Ohara O., Ko M.S.H.
Generation and profiling of 2,135 human ESC lines for the systematic analyses of cell states perturbed by inducing single transcription factors.
Cell Rep. 31:107655.1-107655.23(2020)

Cross-references
Encyclopedic resources Wikidata; Q98132439
Entry history
Entry creation02-Jul-2020
Last entry update10-Sep-2024
Version number7