ID   N27-A
AC   CVCL_ZJ56
DR   Millipore; SCC196
DR   Wikidata; Q98127400
RX   PubMed=27512998;
CC   Characteristics: Improved in vitro model of dopamine neurons for research on Parkinson's disease. Expresses TH and DAT at 4 times the level of the parent cell line. Also expresses VMAT2, dopaminergic transcription factors, dopaminergic storage protein (PubMed=27512998).
CC   Doubling time: ~36 hours (PubMed=27512998).
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P03070; SV40 large T antigen.
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo.
CC   Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) (Note=pSV3-neo).
CC   Derived from site: In situ; Embryonic brain, mesencephalon; UBERON=UBERON_0009616.
OX   NCBI_TaxID=10116; ! Rattus norvegicus (Rat)
HI   CVCL_D584 ! N27
SX   Sex unspecified
AG   12FD
CA   Transformed cell line
DT   Created: 02-07-20; Last updated: 10-09-24; Version: 7
//
RX   PubMed=27512998; DOI=10.1371/journal.pone.0160847; PMCID=PMC4981411;
RA   Gao L., Zhou W.-B., Symmes B., Freed C.R.;
RT   "Re-cloning the N27 dopamine cell line to improve a cell culture model
RT   of Parkinson's disease.";
RL   PLoS ONE 11:e0160847.1-e0160847.18(2016).
//