Cellosaurus CHO-K1 UA10 (CVCL_ZE99)
| Cell line name | CHO-K1 UA10 |
|---|---|
| Synonyms | UA10 |
| Accession | CVCL_ZE99 |
| Resource Identification Initiative | To cite this cell line use: CHO-K1 UA10 (RRID:CVCL_ZE99) |
| Comments | Characteristics: Doubly auxotrophic; deficient in 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase (ATIC) and uridine monophosphate synthetase (UMPS), respectively disrupting the purine and pyrimidine de novo synthesis pathways. Characteristics: Useful for the production of recombinant monoclonal antibodies as it rovides a rapid and efficient selection method for the serial or simultaneous transfer of genes for multiple polypeptides of choice into the cells using readily available purine- and pyrimidine-free commercial media. Knockout cell: Method=CRISPR/Cas9; UniProtKB; G3I1V4; Atic. Knockout cell: Method=CRISPR/Cas9; UniProtKB; G3GSP0; Umps. Derived from site: In situ; Ovary; UBERON=UBERON_0000992. Cell type: Epithelial cell of ovary; CL=CL_2000064. |
| Species of origin | Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) (NCBI Taxonomy: 10029) |
| Hierarchy | Parent: CVCL_0214 (CHO-K1) |
| Sex of cell | Female |
| Age at sampling | Adult |
| Category | Spontaneously immortalized cell line |
| Publications | PubMed=32346859; DOI=10.1002/bit.27367 |
| Cross-references | |
| Encyclopedic resources | Wikidata; Q98125620 |
| Entry history | |
| Entry creation | 02-Jul-2020 |
| Last entry update | 30-Jan-2024 |
| Version number | 5 |