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Cellosaurus LentiPro26-A59 (CVCL_YZ84)

[Text version]
Cell line name LentiPro26-A59
Synonyms LentiPro26-4070A-mCPGFP clone 59
Accession CVCL_YZ84
Resource Identification Initiative To cite this cell line use: LentiPro26-A59 (RRID:CVCL_YZ84)
Comments Characteristics: Transfected with the pGP(T26S)P-blast plasmid containing HIV-1 Gag-Pro(Thr514Ser)-Pol-bsr, the pREV-hygro-WPRE plasmid containing Rev-hph and a woodchuck hepatitis post-transcriptional regulatory element (WPRE), the pMONO-zeo-4070A plasmid containing a ferritin heavy chain promoter fused with SV40 enhancer followed by MLV 4070A env, IRES and ble and the pRRLSIN-mCP-GFP plasmid containing mCherry, pac, the human phosphoglycerate kinase promoter, GFP and WPRE.
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P12493; HIV-1 isolate NY5 gag.
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P12497; HIV-1 isolate NY5 pol (Note=With p.Thr514Ser).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P04325; HIV-1 isolate PCV12 rev.
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; Q89683; Murine leukemia virus isolate 4070A env.
Genetic integration: Method=Transfection/transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
Genetic integration: Method=Transfection/transduction; Gene=FPbase; ZERB6; mCherry (Note=Red fluorescent protein derived from drFP583 (dsRed)).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P33967; B.cereus Bsr.
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P17493; Streptoalloteichus hindustanus ble (BleoR).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00552; Transposon Tn5 neo.
Transformant: NCBI_TaxID; 28285; Adenovirus 5.
Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) (Note=tsA).
Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_YZ83 (LentiPro26-4070A-mCPGFP)
Sex of cell Female
Age at sampling Fetus
Category Transformed cell line
Publications

PubMed=29588490; DOI=10.1038/s41598-018-23593-y; PMCID=PMC5869598
Tomas H.A., Rodrigues A.F., Carrondo M.J.T., Coroadinha A.S.
LentiPro26: novel stable cell lines for constitutive lentiviral vector production.
Sci. Rep. 8:5271-5271(2018)

Cross-references
Encyclopedic resources Wikidata; Q98126898
Entry history
Entry creation02-Jul-2020
Last entry update10-Sep-2024
Version number9