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Cellosaurus CNE1 SRPK1 KO (CVCL_YT53)

[Text version]
Cell line name CNE1 SRPK1 KO
Synonyms SRPK1 KO NPC
Accession CVCL_YT53
Resource Identification Initiative To cite this cell line use: CNE1 SRPK1 KO (RRID:CVCL_YT53)
Comments Problematic cell line: Contaminated. The parent cell line (CNE-1) seems to be a hybrid of HeLa and of cell line of unknown origin.
Knockout cell: Method=CRISPR/Cas9; HGNC; 11305; SRPK1.
Genetic integration: Method=Transfection/transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
Genetic integration: Method=Transfection/transduction; Gene=FPbase; EGE3L; DsRed (Note=drFP583, red fluorescent protein from Discosoma sp.).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_6888 (CNE-1)
Category Hybrid cell line
Publications

PubMed=32671994; DOI=10.1002/cnr2.1224; PMCID=PMC7941449
Prattapong P., Ngernsombat C., Aimjongjun S., Janvilisri T.
CRISPR/Cas9-mediated double knockout of SRPK1 and SRPK2 in a nasopharyngeal carcinoma cell line.
Cancer Rep. 2:e1224.1-e1224.7(2019)

Cross-references
Encyclopedic resources Wikidata; Q93455913
Entry history
Entry creation12-Mar-2020
Last entry update10-Sep-2024
Version number8