Cellosaurus HEK293 CNR1 cAMP-Nomad (CVCL_YI91)
| Cell line name | HEK293 CNR1 cAMP-Nomad |
|---|---|
| Synonyms | CB1 cAMP-Nomad; CB1 Nomad; cAMPNomad-CB1 |
| Accession | CVCL_YI91 |
| Resource Identification Initiative | To cite this cell line use: HEK293 CNR1 cAMP-Nomad (RRID:CVCL_YI91) |
| Comments | Characteristics: In absence of cAMP, the fluorescent biosensor is localized in the cellular membrane. An increase in this second messenger concentration leads to change in the structural folding of Nomad biosensor that promotes its cellular relocation in the vesicular trafficking of the cells. Characteristics: The red cAMP-Nomad biosensor consists of a membrane localization peptide derived from interleukin-2 receptor alpha chain, a TurboFP650 fluorescent protein, a Ht31 peptide fused to a KDEL signal. Genetic integration: Method=Transfection/transduction; Gene=HGNC; HGNC:2159; CNR1. Genetic integration: Method=Transfection/transduction; Gene=FPbase; JS838; TurboFP650 (Note=Far-red fluorescent protein derived from eqFP578). Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR). Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00552; Transposon Tn5 neo. Transformant: NCBI_TaxID; 28285; Adenovirus 5. Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113. |
| Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) |
| Hierarchy | Parent: CVCL_0045 (HEK293) |
| Sex of cell | Female |
| Age at sampling | Fetus |
| Category | Transformed cell line |
| Cross-references | |
| Cell line collections (Providers) | Innoprot; P70527 |
| Encyclopedic resources | Wikidata; Q94092863 |
| Entry history | |
| Entry creation | 19-Dec-2019 |
| Last entry update | 19-Dec-2024 |
| Version number | 10 |