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Cellosaurus CHO-K1 Dd-B7 (CVCL_YD17)

[Text version]
Cell line name CHO-K1 Dd-B7
Synonyms Dd-B7
Accession CVCL_YD17
Resource Identification Initiative To cite this cell line use: CHO-K1 Dd-B7 (RRID:CVCL_YD17)
Comments Characteristics: Lacks Na+-dependent glutamate transport activity.
Characteristics: Selection is effected by tritium suicide selection. Mutagenized cells are bathed in tritiated D-aspartate and stored so as to allow time for cells that had taken up the D-aspartate to be killed by radiation damage.
Selected for resistance to: NCIt; C165595; Tritium beta radiation.
Transformant: ChEBI; CHEBI:23994; Ethyl methanesulfonate (Methylsulfonic acid ethyl ester; MSEE).
Derived from site: In situ; Ovary; UBERON=UBERON_0000992.
Cell type: Epithelial cell of ovary; CL=CL_2000064.
Species of origin Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) (NCBI Taxonomy: 10029)
Hierarchy Parent: CVCL_0214 (CHO-K1)
Children:
CVCL_YD20 (CHO-K1 Dd-B7/GLT-1)
Sex of cell Female
Age at sampling Adult
Category Transformed cell line
Publications

PubMed=8782489; DOI=10.1007/BF02369900
Igo R.P. Jr., Ash J.F.
New mutations and phenotypes associated with glutamate and aspartate transport in Chinese hamster ovary (CHO-K1) cells.
Somat. Cell Mol. Genet. 22:87-103(1996)

PubMed=9498811; DOI=10.1016/S0014-5793(98)00036-2
Levy L.M., Attwell D., Hoover F., Ash J.F., Bjoras M., Danbolt N.C.
Inducible expression of the GLT-1 glutamate transporter in a CHO cell line selected for low endogenous glutamate uptake.
FEBS Lett. 422:339-342(1998)

PubMed=9822723; DOI=10.1523/JNEUROSCI.18-23-09620.1998; PMCID=PMC6793325
Levy L.M., Warr O., Attwell D.
Stoichiometry of the glial glutamate transporter GLT-1 expressed inducibly in a Chinese hamster ovary cell line selected for low endogenous Na+-dependent glutamate uptake.
J. Neurosci. 18:9620-9628(1998)

Cross-references
Encyclopedic resources Wikidata; Q93451720
Entry history
Entry creation19-Dec-2019
Last entry update30-Jan-2024
Version number6