Cellosaurus HEK293-DR-GFP-XRCC2-13/X2+ (CVCL_XX09)
| Cell line name | HEK293-DR-GFP-XRCC2-13/X2+ | ||||||||||||||||||||||||||||||||||||
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| Accession | CVCL_XX09 | ||||||||||||||||||||||||||||||||||||
| Resource Identification Initiative | To cite this cell line use: HEK293-DR-GFP-XRCC2-13/X2+ (RRID:CVCL_XX09) | ||||||||||||||||||||||||||||||||||||
| Comments | Characteristics: The parental XRCC2 KO cell line is functionally reconstituted by retroviral transfection of XRCC2. Characteristics: Transfected with the hprtDR-GFP reporter construct that targets the DR-GFP into the hprt locus and includes a dominant selectable puroR marker. The DR-GFP construct which contains a tandem repeat of two inactive genes coding for EGFP. After transfection with an expression vector coding for the I-SceI enzyme, recombination induced by the cleavage of the I-SceI site recreates a functional EGFP. Characteristics: Transfected with the tetracycline transactivator (tTA) protein, a fusion of tetR with the activation domain of HSV-1 VP16. Doubling time: ~40 hours (DSMZ=ACC-853). Knockout cell: Method=CRISPR/Cas9; HGNC; HGNC:12829; XRCC2. Genetic integration: Method=Transfection/transduction; Gene=HGNC; HGNC:12829; XRCC2. Genetic integration: Method=Transfection/transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP). Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR. Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P06492; Human herpesvirus 1 VP16 (UL28) (Note=With p.Met1_Arg360del). Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR). Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P13249; S.alboniger pac (PuroR). Transformant: NCBI_TaxID; 28285; Adenovirus 5. Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113. | ||||||||||||||||||||||||||||||||||||
| Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) | ||||||||||||||||||||||||||||||||||||
| Hierarchy | Parent: CVCL_XX04 (HEK293-DR-GFP-XRCC2-13) | ||||||||||||||||||||||||||||||||||||
| Sex of cell | Female | ||||||||||||||||||||||||||||||||||||
| Age at sampling | Fetus | ||||||||||||||||||||||||||||||||||||
| Category | Transformed cell line | ||||||||||||||||||||||||||||||||||||
| STR profile | Source(s): DSMZ=ACC-853 Markers:
Run an STR similarity search on this cell line | ||||||||||||||||||||||||||||||||||||
| Cross-references | |||||||||||||||||||||||||||||||||||||
| Cell line collections (Providers) | DSMZ; ACC-853 | ||||||||||||||||||||||||||||||||||||
| Cell line databases/resources | DSMZCellDive; ACC-853 | ||||||||||||||||||||||||||||||||||||
| Encyclopedic resources | Wikidata; Q94095077 | ||||||||||||||||||||||||||||||||||||
| Entry history | |||||||||||||||||||||||||||||||||||||
| Entry creation | 19-Dec-2019 | ||||||||||||||||||||||||||||||||||||
| Last entry update | 19-Dec-2024 | ||||||||||||||||||||||||||||||||||||
| Version number | 11 | ||||||||||||||||||||||||||||||||||||