ID HEK293-DR-GFP-RAD51C-2 AC CVCL_XX02 DR DSMZ; ACC-846 DR DSMZCellDive; ACC-846 DR Wikidata; Q94095050 CC Characteristics: Transfected with the hprtDR-GFP reporter construct that targets the DR-GFP into the hprt locus and includes a dominant selectable puroR marker. The DR-GFP construct which contains a tandem repeat of two inactive genes coding for EGFP. After transfection with an expression vector coding for the I-SceI enzyme, recombination induced by the cleavage of the I-SceI site recreates a functional EGFP. CC Characteristics: Transfected with the tetracycline transactivator (tTA) protein, a fusion of tetR with the activation domain of HSV-1 VP16. CC Doubling time: ~40 hours (DSMZ=ACC-846). CC Knockout cell: Method=CRISPR/Cas9; HGNC; HGNC:9820; RAD51C. CC Genetic integration: Method=Transfection/transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP). CC Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR. CC Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P06492; Human herpesvirus 1 VP16 (UL28) (Note=With p.Met1_Arg360del). CC Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P13249; S.alboniger pac (PuroR). CC Transformant: NCBI_TaxID; 28285; Adenovirus 5. CC Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113. ST Source(s): DSMZ=ACC-846 ST Amelogenin: X ST CSF1PO: 12 ST D13S317: 12,14 ST D16S539: 9,13 ST D18S51: 17,18 ST D19S433: 18 ST D21S11: 30.2 ST D2S1338: 19 ST D3S1358: 15,17 ST D5S818: 8 ST D7S820: 11,12 ST D8S1179: 12,14 ST FGA: 23 ST Penta D: 9 ST Penta E: 7,15 ST TH01: 7,9.3 ST TPOX: 11 ST vWA: 16,19 OX NCBI_TaxID=9606; ! Homo sapiens (Human) HI CVCL_XX00 ! HEK293-DR-GFP SX Female AG Fetus CA Transformed cell line DT Created: 19-12-19; Last updated: 19-12-24; Version: 10 //