ID   MHHi006-A-1
AC   CVCL_XI78
SY   CFTRdTomato74; hHSC_F1285_T-iPS2-CFTRTomato/wt C74; HSC1285iPS2_CFTRTomatoK74
DR   hPSCreg; MHHi006-A-1
DR   Wikidata; Q95989462
RX   PubMed=31080112;
RX   PubMed=31473565;
CC   From: Hannover Medical School, LEBAO (MHH); Hannover; Germany.
CC   Characteristics: Using TALEN a ZeoR-T2A-dTomato(nuc) construct was integrated in frame in front of the start codon of one of the two alleles of CFTR (PubMed=31473565).
CC   Genetic integration: Method=TALEN; Gene=FPbase; PGG5S; tdTomato (Note=Bright red fluorescent protein derived from drFP583 (DsRed)).
CC   Genetic integration: Method=TALEN; Gene=UniProtKB; P17493; Streptoalloteichus hindustanus ble (BleoR).
CC   Derived from site: In situ; Umbilical cord blood; UBERON=UBERON_0012168.
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_VN33 ! MHHi006-A
SX   Male
AG   <1D
CA   Induced pluripotent stem cell
DT   Created: 19-12-19; Last updated: 10-09-24; Version: 6
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RX   PubMed=31080112; DOI=10.1016/j.stemcr.2019.04.014; PMCID=PMC6565754;
RA   Merkert S., Schubert M., Olmer R., Engels L., Radetzki S., Veltman M.,
RA   Scholte B.J., Zollner J., Pedemonte N., Galietta L.J.V.,
RA   von Kries J.P., Martin U.;
RT   "High-throughput screening for modulators of CFTR activity based on
RT   genetically engineered cystic fibrosis disease-specific iPSCs.";
RL   Stem Cell Reports 12:1389-1403(2019).
//
RX   PubMed=31473565; DOI=10.1016/j.scr.2019.101542;
RA   Engels L., Olmer R., de la Roche J., Gohring G., Ulrich S., Haller R.,
RA   Martin U., Merkert S.;
RT   "Generation of a CFTR knock-in reporter cell line (MHHi006-A-1) from a
RT   human induced pluripotent stem cell line.";
RL   Stem Cell Res. 40:101542-101542(2019).
//