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Cellosaurus A2058-RFP/H2B-GFP BrM (CVCL_XH74)

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Cell line name A2058-RFP/H2B-GFP BrM
Synonyms A2058(RFP/H2B-GFP) BrM
Accession CVCL_XH74
Resource Identification Initiative To cite this cell line use: A2058-RFP/H2B-GFP BrM (RRID:CVCL_XH74)
Comments Part of: Brain metastasis cell lines panel.
Population: Caucasian.
Characteristics: Brain parenchymal space metastatic derivative of parental cell line. Established after 3 passages in NMRI-nu/nu mice 4 weeks after intra-cardiac injection.
Characteristics: The H2B-eGFP fusion construct is under the control of the pPGK promoter and localizes to the nucleus.
Transfected with: HGNC; 4761; H2BC11 (with p.Gly26Asp and p.Ile119Val and fused in frame with eGFP).
Transfected with: UniProtKB; P42212; GFP (with p.Phe64Leu, p.Ser65Thr and p.His231Leu = EGFP).
Transfected with: tdTomato, a bright red fluorescent protein derived from drFP583 (DsRed).
Derived from site: Metastatic; Lymph node; UBERON=UBERON_0000027.
Sequence variations
  • Mutation; HGNC; 1097; BRAF; Simple; p.Val600Glu (c.1799T>A); ClinVar=VCV000013961; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 11730; TERT; Simple; c.1-124C>T (c.228C>T) (C228T); Zygosity=Unspecified; Note=In promoter (from parent cell line).
  • Mutation; HGNC; 11998; TP53; Simple; p.Val274Phe (c.820G>T); ClinVar=VCV000376674; Zygosity=Homozygous (from parent cell line).
Disease Amelanotic melanoma (NCIt: C3802)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_XH73 (A2058-RFP/H2B-GFP)
Sex of cell Male
Age at sampling 43Y
Category Cancer cell line
Web pages https://apps.cnio.es/app/BrainMetastasis/CellLines

PubMed=27521448; DOI=10.1158/1078-0432.CCR-16-1327
Osswald M., Blaes J., Liao Y.-X., Solecki G., Gommel M., Berghoff A.S., Salphati L., Wallin J.J., Phillips H.S., Wick W., Winkler F.
Impact of blood-brain barrier integrity on tumor growth and therapy response in brain metastases.
Clin. Cancer Res. 22:6078-6087(2016)

Cell line databases/resources cancercelllines; CVCL_XH74
Encyclopedic resources Wikidata; Q93311456
Entry history
Entry creation19-Dec-2019
Last entry update05-Oct-2023
Version number9