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Cellosaurus U87.CD4.CCR5 (CVCL_X630)

[Text version]
Cell line name U87.CD4.CCR5
Synonyms U87/CD4/CCR5; U87.CD4-CCR5; U87 CD4+CCR5+
Accession CVCL_X630
Resource Identification Initiative To cite this cell line use: U87.CD4.CCR5 (RRID:CVCL_X630)
Comments Problematic cell line: Misidentified. Grand-parent cell line (U-87MG ATCC) is not the original glioblastoma cell line established in 1968 at the University of Uppsala.
Population: Caucasian.
Genetic integration: Method=Transfection/transduction; Gene=HGNC; 1678; CD4.
Genetic integration: Method=Transfection/transduction; Gene=HGNC; 1606; CCR5.
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00552; Transposon Tn5 neo.
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
Sequence variations
  • Mutation; HGNC; 7765; NF1; Simple; p.Phe1247Ilefs*18 (c.3739_3742delTTTG) (3737_3740delTGTT); ClinVar=VCV000420078; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 9588; PTEN; Simple; c.209+1G>T; ClinVar=VCV000229705; Zygosity=Homozygous; Note=Splice donor mutation (from parent cell line).
  • Mutation; HGNC; 11730; TERT; Simple; c.1-124C>T (c.228C>T) (C228T); Zygosity=Unspecified; Note=In promoter (from parent cell line).
Disease Glioblastoma (NCIt: C3058)
Glioblastoma (ORDO: Orphanet_360)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_X626 (U87.CD4)
Sex of cell Male
Age at sampling Age unspecified
Category Cancer cell line
Web pages http://www.nibsc.org/Default.aspx?pageid=597&id=0072&theme=default
Publications

PubMed=9311827; DOI=10.1128/JVI.71.10.7478-7487.1997; PMCID=PMC192094
Bjorndal A., Deng H.-K., Jansson M., Fiore J.R., Colognesi C., Karlsson A., Albert J., Scarlatti G., Littman D.R., Fenyo E.-M.
Coreceptor usage of primary human immunodeficiency virus type 1 isolates varies according to biological phenotype.
J. Virol. 71:7478-7487(1997)

PubMed=15169555; DOI=10.1186/1742-4690-1-2; PMCID=PMC416571
Princen K., Hatse S., Vermeire K., De Clercq E., Schols D.
Establishment of a novel CCR5 and CXCR4 expressing CD4+ cell line which is highly sensitive to HIV and suitable for high-throughput evaluation of CCR5 and CXCR4 antagonists.
Retrovirology 1:2.1-2.13(2004)

Cross-references
Cell line collections (Providers) BEI_Resources; ARP-4035
Cell line databases/resources cancercelllines; CVCL_X630
Encyclopedic resources Wikidata; Q54973767
Entry history
Entry creation05-Sep-2014
Last entry update10-Sep-2024
Version number15