Cellosaurus logo
expasy logo

Cellosaurus SpESF (CVCL_WS48)

[Text version]
Cell line name SpESF
Synonyms SpEEE Serum-Free
Accession CVCL_WS48
Resource Identification Initiative To cite this cell line use: SpESF (RRID:CVCL_WS48)
Comments Group: Hybridoma fusion partner cell line.
Group: Patented cell line.
Group: Serum/protein free medium cell line.
Characteristics: Transfected with a hBCL2-EEE-pZeoSV2+ construct.
Selected for resistance to: ChEBI; CHEBI:63486; 8-azaguanine.
Genetic integration: Method=Transfection/transduction; Gene=HGNC; 990; BCL2 (Note=With p.Thr69Glu, p.Ser70Glu and p.Ser87Glu = BCL2-EEE).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P17493; Streptoalloteichus hindustanus ble (BleoR).
Disease Mouse plasma cell myeloma (NCIt: C125417)
Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Hierarchy Parent: CVCL_WS47 (SpEEE)
Children:
CVCL_WS49 (SpESFX)
Sex of cell Female
Category Cancer cell line
Publications

Patent=US7608425
Goldenberg D.M., Chang C.-H., Rossi E.A., Nordstrom D.
Methods for protein expression in mammalian cells in serum-free medium.
Patent number US7608425, 27-Oct-2009

PubMed=21473000; DOI=10.1002/btpr.584
Rossi D.L., Rossi E.A., Goldenberg D.M., Chang C.-H.
A new mammalian host cell with enhanced survival enables completely serum-free development of high-level protein production cell lines.
Biotechnol. Prog. 27:766-775(2011)

Cross-references
Encyclopedic resources Wikidata; Q98132814
Entry history
Entry creation05-Jul-2019
Last entry update10-Sep-2024
Version number5