Cellosaurus H1 iCas9 (CVCL_WS14)
Cell line name | H1 iCas9 | ||||||||||||||||||||||||||||||||
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Accession | CVCL_WS14 | ||||||||||||||||||||||||||||||||
Resource Identification Initiative | To cite this cell line use: H1 iCas9 (RRID:CVCL_WS14) | ||||||||||||||||||||||||||||||||
Comments | From: Memorial Sloan Kettering Cancer Center; New York; USA. Characteristics: Cell line with robust Dox-inducible expression of Cas9. Can be used for rapid and highly efficient generation of biallelic knockout hPSCs for loss-of-function studies. Using TALEN two constructs SA-2A-Hygro-Cas9-TRE and SA-2A-Neo-M2rtTA were introduced in the AAVS1 safe harbor locus, one in each allele. Genetic integration: Method=TALEN; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9. Genetic integration: Method=TALEN; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR). Genetic integration: Method=TALEN; Gene=UniProtKB; P00552; Transposon Tn5 neo. Miscellaneous: STR profile from personal communication of Huangfu, Dan-Wei. Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358. Cell type: Embryonic stem cell; CL=CL_0002322. | ||||||||||||||||||||||||||||||||
Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) | ||||||||||||||||||||||||||||||||
Hierarchy | Parent: CVCL_9771 (WA01) | ||||||||||||||||||||||||||||||||
Sex of cell | Male | ||||||||||||||||||||||||||||||||
Age at sampling | Blastocyst stage | ||||||||||||||||||||||||||||||||
Category | Embryonic stem cell | ||||||||||||||||||||||||||||||||
STR profile | Source(s): Direct_author_submission Markers:
Run an STR similarity search on this cell line | ||||||||||||||||||||||||||||||||
Publications | PubMed=28196600; DOI=10.1016/j.stem.2017.01.001; PMCID=PMC5419850 PubMed=31110351; DOI=10.1038/s41588-019-0408-9; PMCID=PMC6545159 | ||||||||||||||||||||||||||||||||
Cross-references | |||||||||||||||||||||||||||||||||
Encyclopedic resources | Wikidata; Q93935734 | ||||||||||||||||||||||||||||||||
Entry history | |||||||||||||||||||||||||||||||||
Entry creation | 05-Jul-2019 | ||||||||||||||||||||||||||||||||
Last entry update | 10-Sep-2024 | ||||||||||||||||||||||||||||||||
Version number | 9 |